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Bacterial communities of Beijing surface waters as revealed by 454 pyrosequencing of the 16S rRNA gene

机译:16S rRNA基因的454焦磷酸测序揭示了北京地表水的细菌群落

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摘要

As a better understanding of Beijing surface water ecosystems can provide clues for environmental management and public health, here, we report a study of the bacterial communities of five Beijing surface waters conducted using 454 pyrosequencing of 16S ribosomal RNA (rRNA) genes. We expected to observe a core bacterial community among the surface waters and differences in bacterial community abundance over the different locations of sampling. In this study, we obtained a total of 60,810 trimmed reads from the five samples after the removal of unqualified reads. Bacterial sequences from the five samples were classified into taxonomic classes using the default settings of the mothur platform. Our results provided insight into the bacterial community composition of surface waters and revealed that there was a core microbial community in the microbial populations of surface samples at different geographic locations, with 13 phyla and 40 genera in common. Our findings also revealed the differences in bacterial communities among five surface water samples obtained at different locations.
机译:为了更好地了解北京地表水生态系统可以为环境管理和公共卫生提供线索,在这里,我们报告了一项对15个北京地表水细菌群落的研究,该研究使用454基因进行16S核糖体RNA(rRNA)基因的焦磷酸测序。我们期望在地表水域中观察到一个核心细菌群落,并且在不同采样地点细菌群落丰度也有所不同。在这项研究中,我们从五个样本中剔除了不合格的读数后,总共获得了60,810个修剪后的读数。使用mothur平台的默认设置,将五个样本中的细菌序列分类为分类学类别。我们的结果提供了对地表水细菌群落组成的见解,并揭示了在不同地理位置的地表样品微生物种群中存在一个核心微生物群落,共有13个门和40个属。我们的发现还揭示了在不同位置获得的五个地表水样品中细菌群落的差异。

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